Impact of eugenol on in vivo model of 6-hydroxydopamine-induced oxidative stress.

Oxidative stress is involved in many pathological disturbs, such as neurodegenerative disorders. Eugenol (Eug) is a phenolic compound with antioxidant and neuroprotective activities. Then, the present study was conducted to investigate the potential neuroprotective effects of Eug on oxidative stress model induced by 6-hydroxydopamine (6-OHDA) in rats. Firstly, the in vivo oxidative stress model was performed by intrastriatal injection (int.) of 6-OHDA (21 µg), followed by the treatment of Eug (0.1, 1 and 10 mg/kg/7 days) per os (p.o.). On the 7 th day, behavioural tests were performed. On the 8 th day, all the animals were euthanasied and their cerebral areas were excised for neurochemical and transcriptional analyses. The results showed that the treatment with Eug promoted neuroprotective effects on in vivo through reducing of oxidative stress and modulation of genes related to antioxidant activity. Furthermore, animals treated with Eug demonstrated returning of behavioural performance and body weight gain to normal conditions. Thus, the present study reports the neuroprotective effects of Eugenol against oxidative stress induced by 6-hydroxydopamine in rats.