Determination of HLA‐A*02 alleles using nested PCR‐SSP in Korean population

HLA‐A2 is one of the most diversified HLA‐class I antigen with 17 subtypes so far identified at the molecular level. HLA‐A*02 subtyping has significant implications on the tissue typing for organ and bone marrow transplantations. Recently, DNA‐based typing methods have been successfully applied to the elucidation of HLA gene polymorphisms. In the present study, HLA‐A*02 genotyping was established by using nested polymerase chain reaction‐sequence specific primers (PCR‐SSP) and distribution of A*02 alleles were determined in Korean individuals. Genomic DNA prepared from four B‐lymphoblastoid cell lines and lymphocytes from serologically defined 48 HLA‐A2 Korean individuals by phenol/chloroform extractions was typed. The results of the four B‐lymphoblastoid cells were consistant with the previous data typed by PCR analysis. Five A*02 alleles‐A*0201, A*0203, A*0206, A*0207 and A*0210 ‐ were commonly observed in a total of 17 A*02 alleles. Of these, A*0207 (f = 49.0%) was the most frequent allele in Korean po...