Waking Electroencephalogram Activity as a Consequence of Sleep and Total Sleep Deprivation in the Rat

Effects of 6 hours of total sleep deprivation (TSD) by gentle handling and 6 hours of sleep on the waking electroencephalogram (EEG) activity of 14 Wistar rats were studied during the lights-on portion of the light-dark cycle under two TSD schedules: a) TSD 0800-1400 hours and sleep 1400-2000 hours, and b) sleep 0800-1400 hours and TSD 1400-2000 hours. EEG was recorded monopolarly from left and right parietals referred to ipsilateral reference electrodes. Spectral analysis was performed on samples of waking EEG during TSD (each 30 minutes) and during sleep (each 60 minutes after enforced awakening). The following significant changes were observed: TSD induced a linear increase in the absolute power of delta (1.46-3.42 Hz) and the full band (1.6-24.9 Hz) and produced a decrease in the interparietal correlation of theta. Sleep induced the opposite results. Theta relative power (power in a band expressed as a percentage of total power between 1.46 and 24.9 Hz) showed a circadian effect. It was higher at 1400 hours than at 0800 and 2000 hours after both sleep and TSD conditions. Six hours of TSD were enough to induce significant changes in the waking EEG regardless of position of TSD in the lights-on period of the light-dark cycle.