Platelets-released insulin-like growth factor 1 is correlated with anxiety in myocardial infarction

Abstract Objective The aim of this study is to investigate the role of platelets in anxiety in myocardial infarction (MI). Methods A protein kinase D (PKD) inhibitor CRT0066101 was administrated to MI rat models. Open-field (OF) test, dark-light transfer (DLT) test and elevated plus maze (EPM) test were used to assess anxiety of animals. Platelets granule releasing was evaluated by ATP releasing assay and flow cytometry detecting CD62P. ELISA was used to measure the levels of vWF, soluble tissue factor (sTF) and IGF1 in serum. Protein phosphorylations were evaluated with western blotting. 171 patients with MI, 189 patients with stable CAD (SCAD) and 200 healthy volunteers (HV) were included in the population study. HAM-A was used to evaluate the anxiety. Results In the animal study, PKD inhibitor significantly suppressed platelets granule releasing and serum IGF1 levels (P  Conclusions Anxiety is exacerbated in MI via endothelial damage-induced platelets granule releasing-mediated IGF1.