WBC differential in Gir and Jaffarabadi animals at different physiological stages

A study was conducted to establish the leucogram in Gir cattle and Jaffarabadi buffaloes at different age’s viz.1 wk, 1m, 3m, 6m, 12m, 24m and 36m, in lactating (1m, 2m, 3m) and non-lactating (pregnant and non-pregnant) and in castrated males and bulls. Thin blood smears were made and stained with Eosin and Methylene Blue (Field Stain) and the different types of leucocytes (DLC) were enumerated. The percentage of neutrophils in the blood smear is more than the percentage of lymphocytes at 1 wk age, indicating the pre-ruminant status of the animal. However, from one month of age onwards it was observed that the percentage of lymphocytes was more than the percentage of neutrophils as seen in ruminant animal.