Broyler Tavuklarda Mersin, Biberiye ve Kekik Esansiyel Yağlarının Bağırsak Motilitesi Üzerine Etkisi

This research was conducted to determine the effects of essential oils of myrtle, rosemary, and thyme, which are common essential oils in broiler nutrition, on small intestine contractility. For this purpose, small intestines of 10 broiler chickens of 40-42 days old, which were sacrificed in a special slaughterhouse and were put into Krebs solution (NaCl, 118 mmol/l; KCl, 4.7 mmol/l; CaCl2, 2.5 mmol/l; MgSO4, 1 mmol/l; KH2PO4, 1 mmol/l glucose 11, mmol/l; NaHCO3, 25 mmol/l) and transferred to the laboratories at +4 0C. Isolated strips of duodenum, jejunum and ileum were placed in a four-channeled, isolated organ bath system at 39 °C, ventilated by a mixture of 95% O2 - %5 CO2 regularly. The contractions of the tissues were recorded by displaying them on the computer with the help of "force transducer" and "acquisition system". The effective doses and effects of essential oils of myrtle, rosemary, and thyme, obtained by hydrodistillation method of Clevenger apparatus, were determined in a concentration range of 0.1-1000 μg / ml. Similarly, maximal contractions were obtained by applying EFS at acetylcholine concentrations in a range of 10-10 -10-3 M. The maximal effective doses of all three essentil oils were re-evaluated at acetylcholine and EFS applications in which maximal contractions are obtained. It was determined that the essential oils of 1000 μg/ml of rosemary and thyme, and 300 and 1000 μg/ml levels of myrtle inhibited spontaneous contractions of all three tissues completely, whereas no significant responses were observed in these tissues at other concentration levels. In tissues stimulated by acetylcholine and EFS, the effective doses of the essential oils inhibited contractions in the range of 15-35%. In conclusion, the essential oils of myrtle, rosemary, and thyme were demonstrated to inhibit the contractions of small intestine in vitro . However, it has been observed that higher doses are needed to be applied to obtain this effect.