One-pot degradation of urine wastewater by combining simultaneous halophilic nitrification and aerobic denitrification in air-exposed biocathode microbial fuel cells (AEB-MFCs)

Abstract Urine wastewater is used as fuel in microbial fuel cells to generate power for several applications. However, the knowledge on the removal efficiencies of pollutants and bacterial composition of electrode biofilm is still lacking. In this study, two air-exposed biocathode microbial fuel cells (AEB-MFCs) were constructed and some nitrogen-removing consortium were inoculated to fabricate multifunctional AEBs for urine treatment and energy recovery. Results demonstrated that urine wastewater can be degraded through one-pot degradation without positive aeration. The removal efficiencies of NH4+-N, total nitrogen and chemical oxygen demand reached 86.8% ± 1.5%, 62.7% ± 2.3%, and 52.7% ± 1.6% respectively. Cyclic voltammetry illustrated several catalytic activities related to C/N metabolism occurred in both biofilms and varied with the operation continuing in a single stable cycle. In addition, the community structure analysis revealed that many active microorganisms, including nitrogen-removing bacteria, heterotrophs, and electrochemically active bacteria were enriched in both electrodes, especially many halophilic nitrifiers/denitrifiers occupied in AEBs and directed the system toward the integrated pathways of halophilic nitrogen removal and energy recovery. This study presented a novel method for the energy conversion and effective degradation of urine, which can serve as a promising technology for urine wastewater treatment.