Expression and correlation of PKCζ, MMP-2 and MMP-9 in breast cancer

Purpose To investigate the expressions of PKCζ,MMP-2,and MMP-9 in breast cancer and the relationship with the invasion and metastasis of breast cancer. Methods The expression of PKCζ,MMP-2 and MMP-9 in 100 cases with breast cancer was assessed with immunohistochemistry PV 9000 method. PKCζ-siRNA was transfected into MDA-MB-231 cell lines,called siPKCζ /MDAMB-231. While siRNA construct containing a scrambled sequence was transfected into MDA-MB-231 cells to generate control cells,which were designated as Scr /MDA231 cells. Western blotting was used to measure the expression of PKCζ in transfected cells,and the Transwell invasion assay was used to detect the invasion ability in vitro. The content of MMP-2,MMP-9 were measured by ELISA.Results The expression rates of PKCζ,MMP-2 and MMP-9 in breast cancer tissues were 62. 5%,37. 5% and 32. 5%,and there were significant differences among them( P 0. 05). The expression of PKCζ was much higher than those in the normal breast tissues nearby. The expression of PKC protein was assoiated with lymph node metastasis,distant metastasis( P 0. 01),but was not correlated with other clinicopathologic parameters,such as age,tumor size,histological type,ER,PR,and so on( P 0. 05). The expression of PKCζ,MMP-2 and MMP-9 were lower in siPKCζ / MDA-MB-231 group than those in scr / MDA-MB-231 group,and the in vitro invasion ability was significantly decreased( P 0. 05). Conclusions PKCζ can promote the invasion and metastasis of breast cancer,and correlated with the expression of MMP-2,MMP-9( P 0. 05).