Direct Organogenesis and Plant Regeneration in Preconditioned Tissue Cultures of Lathyrus cicera L., L. ochrus (L.)DC. and L. sativus L.

This study demonstrates the importance of preconditioning of source tissue in regeneration of multiple shoot buds from several species of Lathyrus. Preconditioned multiple shoots of Lathyrus cicera L., L. ochrus (L.)DC. and L. sativus L. were obtained by germinating seeds on Murashige and Skoog (MS) medium containing 50 μM N 6 -benzylaminopurine (BAP) for 2 to 3 weeks. Multiple shoot bud formation occurred when epicotyl explants of preconditioned shoots were cultured on MS medium containing 5-50 μM BAP