Simultaneous determination of insecticides, acaricides and fungicides by thin-layer chromatography

Abstract A method is described for the determination of residual amounts of some insecticides, acaricides and fungicides in fresh and processed fruits. Optimum conditions were established for the extraction of eighteen pesticides, purification of the extracts by column chromatography and subsequent two-dimensional thin-layer chromatography on silica gel GF 254 with cyclohexane-acetone (10:1) and light petroleum—benzene—ethanol (65:30:5) as solvents and with detection under UV light at 254 and 366 nm followed by a 0.1% solution of bromophenol blue.