Construction of human small cell lung cancer animal model of macaca fascicularis

OBJECTIVE: To set up SCLC animal model which was similar to human beings.METHODS: Macaca fascicularis were used as in vivo model.Macaca fasciculari model of SCLC was induced by directly injecting human SCLC cell lines Nci-h446 into the bronchial mucosa through bronchoscopy after spleen resection.This model was successfully established and determined by chest CT scan and bronchoscopy biopsy.RESULTS:The time of a successful macaca fasciculari model of SCLC needed at least 9 months,but the earliest subject showed pathological changes of dyskaryosis was about 6 months.The life state and biochmical level and imaging change of the macaca fasciculari model of SCLC was similar to that of human lung cancer.The successful rate of SCLC model was 50%,namely 3 in 6 macaca fascicularis.In the 12 sites injected with 1×107 cells mL-1,the successful rate was 16.7%,namely 3 in 12 sites;while for those injection with 1×105 or 1×106 cells mL-1,all failed.Among those sites implanted with 1×107 cells mL-1,41.7% subjects(5 sites) showed pathological changes of dyskaryosis or worse(including canceration).The average percentage of successful establishment for all sites injected with cells was 8.3%;13.9% sites showed pathological changes of dyskaryosis or worse(including canceration) in all sites.CONCLUSION: Macaca fascicularis model of SCLC can be induced by directly injecting human SCLC cell lines Nci-h446 into the animal's bronchial mucosa through bronchoscopy,which requires the cell concentration more than 1×107 mL-1.