Assay of hydroxyfarrerol in biological fluids

Abstract A high-performance liquid chromatographic method for the determination of hydroxyfarrerol (IdB 1031) in biological samples was developed. IdB 1031 was first extracted by liquid—solid partition and the extracts were evaporated and analysed on a reversed-phase column under isocratic conditions, using either an electrochemical or a UV detector. The detection limit was ca. 5 ng/ml. Preliminary pharmacokinetic data showed that rats treated orally with 500 mg/kg had an average peak plasma concentration ( C max ) of 497 ng/ml after 2 h.