Geographical classification of Adhatoda vasica based on HPLC fingerprint analysis coupled with multivariate analysis

Adhatoda vasica (Nees) of family acanthaceae is a shrub growing throughout the Indian cape and is valued for having alkaloids with bronchodilation activity. Both seasonal and geographical variation is witnessed for the chemical constituents of the plant. A steadfast and comprehensive method for classifying the origin and assessing the quality of Adhatoda vasica has been developed. The method is based on analysis of HPLC fingerprints, combined with principal component analysis (PCA). Sixteen samples of Adhatoda vasica, collected from different origin of India, were used to establish the fingerprints and 15 peaks were selected for the analysis. PCA classified all the 16 samples into two groups. This method was successfully used to identify the geographical origin of the Adhatoda vasica samples whether from Gujarat or not and to evaluate their quality.