The Cytotoxic and ACE-inhibitory Activities of Promod 278P Hydrolysate of Ovotransferrin

The objective of this study was to investigate the cytotoxic and ACE-inhibitory activities of ovotransferrin and its promod 278P enzyme hydrolysate. Ovotransferrin from egg white was hydrolyzed using promod 278P at 45 °C for 3 h. The cytotoxicity and ACE-inhibitory activity was determined. The promod 278P hydrolysate of ovotransferrin showed a potent cytotoxicity (> 90%) at 20 mg/mL in all cancer cell lines tested. The IC50 value of the promod 278P hydrolysate of ovotransferrin was 1.53 ± 0.20 mg/mL. The promod 278P hydrolysate of ovotransferrin showed a strong ACE-inhibiting activity, but ovotransferrin did not. This result indicated that the promod 278P hydrolysate of ovotransferrin have a great potential as an anticancer and antihypertension agent for humans, but the information on the peptides responsible for the functional activities is not available yet.