Peptostreptococcus faecalis sp. Nov., New Bacterial Species Isolated from Healthy Indigenous Congolese Volunteer

Microbial culturomics is a technique that has become the gold standard method for bacterial identification in clinical microbiology laboratories and for the study of human microbiota, using 18 culture conditions to explore the broadest possible diversity for each sample. This approach allowed us to culture a new bacterial species isolated from a stool sample from a healthy indigenous Congolese volunteer. Strain Marseille-P4308 is a Gram-positive coccus shaped bacterium that optimally grows at 37°C with negative catalase and oxidase. The 16S rRNA gene sequence of the strain has a 96.2% sequence similarity to Peptostreptococcus anaerobius strain NCTC 11460T (GenBank accession number: NR_042847.1). The genome of the strain Marseille-P4308 T has a length of 2,145,294 bp with G+C content of 30.4 mol%. Based on phenotypic, biochemical, genomic and phylogenetic analysis, strain Marseille-P4308T (CSUR P4308T = CECT 9960) clearly appears to be a new species for which the name Peptostreptococcus faecalis sp. nov., is proposed.