A modified method for isolating human quiescent pancreatic stellate cells

Fu Tao Meng,Mei Huang,Fang fang Fan,Feng Shao,Chao Wang,Qiang Huang

A modified method for isolating human quiescent pancreatic stellate cells

2019

Fu Tao Meng
Mei Huang
Fang fang Fan
Feng Shao
Chao Wang
Qiang Huang

Background This study explored a simple, high-yield method for isolating quiescent human pancreatic stellate cells (PSCs) to provide sufficient and reliable raw materials for PSC-related studies.

Keywords:

Cell biology
Hepatic stellate cell
Chemistry
Percoll
Viability assay
Molecular biology
Staining
Oil Red O
Cell culture
Differential centrifugation
Immunofluorescence

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