Differential expression of proteins in Listeria monocytogenes under thermotolerance-inducing, heat shock, and prolonged heat shock conditions.

Abstract Listeria monocytogenes is a foodborne pathogen capable of employing stress adaptive responses to evade a variety of stressors including temperature stress. We employed two-dimensional gel electrophoresis coupled with matrix-assisted laser desorption/ionization–time of flight analysis to study the differential expression of L. monocytogenes (ATCC 43256) soluble proteins at heat shock (60°C) conditions, prolonged heat shock (60°C for 9 minutes) conditions, and thermotolerance-inducing (48°C for 30 minutes followed by 60°C for 9 minutes) conditions. We compared the proteome of L. monocytogenes under these conditions to the proteome at 37°C. Eighteen proteins were differentially expressed at 60°C (6 up-regulated and 12 down-regulated), 21 proteins were differentially expressed (12 up-regulated and 9 down-regulated) when the cells were exposed to 60°C for 9 minutes, and 20 proteins were differentially expressed (10 up-regulated and 10 down-regulated) when cells were initially exposed to 48°C for 30 mi...