Primary study on determination method of free amino acids in the pupa protein hydrolyzate

Preparation of amino acid products by hydrolysis silkworm chrysalis is one of the most effective ways to develop the rich silkworm chrysalis resources in China.In order to control the technological process,the hydrolysis free amino acid contents were detected in the silkworm chrysalis hydrolysis process.This paper summarized several test methods for the detection of total amino acids and each of the free acid content,the double indicator formaldehyde titration,the potential titration,amino acid autoanalyzer,high performance liquid chromatography coupled with a photo diode array detector and an evaporative light scattering detector(HPLC-DAD-ELSD) were employed in the determination.