Correlation between the surface hydrophobicities and elution orders of elapid neurotoxins and cardiotoxins on hydrophobic-interaction high-performance liquid chromatography.

Abstract G. Osthoff , A. I. Louw and C. J. Reinecke . Correlation between the surface hydrophobicities and elution orders of Elapid neurotoxins and cardiotoxins on hydrophobic-interaction high-performance liquid chromatography. Toxicon 26, 475 – 483, 1988 — Hydrophilic and hydrophobic regions were predicted for Elapid neuro- and cardiotoxins. The contribution of these regions to the retention times of neuro- and cardiotoxins on hydrophobic-interaction HPLC was assessed from the known surface accessibilities of amino acid side-chains within these regions. Differences in retention times between neuro- and cardiotoxins on hydrophobic-interaction HPLC could be attributed to differences in hydrophobicity of regions 6 – 12 and 22 – 26 between these two types of toxins. Smaller differences in retention times between cardiotoxins were due to the variable hydrophobicities of regions 1 – 4 and 26 – 36.