Establishment of a PCR Method for the Identification of Mink-Derived Components in Common Edible Meats

2021 
In this study, we established a DNA extraction method of meats and a PCR method for the detection of mink-derived components in common edible meats. The DNA of meats was extracted by a developed mink-derived component detection method and salting-out method, the specific primers of Cyt-b were designed, and the mink-derived components in common edible meats were identified by PCR. The results showed that the concentration and purity of mink meat DNA extracted by the two methods could meet the requirements of PCR. The mink meat DNA extracted by the developed mink DNA detection method had a high purity and yield. The mink-derived DNA in common edible meats could be identified. The specificity of mink meat DNA primer was proved to be good by repeated tests. The developed mink DNA detection method has the advantages of rapid detection and simple operation, which can be used for the identification of meat products in market and provide an experimental basis for the quality control of meat products.
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