MLVA typing of Brucella canis isolated from dogs in Gyeongbuk province, Korea

Abstract Canine brucellosis is the zoonosis in worldwide and Brucella (B.) canis is a facultative intracellular pathogen that has a very limited host. MLVA-16 (Multilocus VNTR analysis) is a efficient method for genotyping of Brucella species. Various methods have been established for genotyping of Brucella species, but most of ana-lytical method is lack reproducibility and limited capability to differentiate them. B. canis isolates (n=73) from 7 farms in Gyeongbuk province in 2003∼2010 were analyzed using 16 VNTR loci. Automatic electro-phoresis system was utilized for more high throughput and rapid simple discrimination. Thirty two genotypes were identified from 73 B. canis isolates. MLVA could contribute to molecular typing for epidemiological evaluation of canine brucellosis. Key words : Brucella canis , VNTR, MLVA, Genotype 서 론 Brucella ( B .) canis 는 미국의 비글견 번식장에서 발생한 유산증의 원인을 조사하던 중 유산태아에서 처음 분리되었고, 이후 1968년 균의 생화학적 특성을 조사하여 B. canis 라고 정식 명명되었다(Carter와 Wise, 2004; Metcalf 등, 1994).