Effect of chondroitin sulfate C on sperm capacitation and fertilization parameters in vitro in pigs

Abstract The objective of this study was to determine the effects of chondroitin sulfate C (CS-C) on sperm capacitation and fertilization parameters in vitro in pigs. Frozen–thawed ejaculated pig sperm (semen S-484) were incubated with fertilization medium containing CS-C (0–2 mg/ml) for 1 h and the capacitation rate with chlorotetracycline (CTC) assay was examined, which showed that CS-C increased the rate of incapacitation F pattern spermatozoa converted to capacitation B pattern sperm cell in concentration-dependent manner and mostly increased capacitation B pattern sperm cell and decreased acrosome reaction AR pattern sperm cell in 1 mg/ml concentration. When sperm was incubated for 1, 2 and 4 h in fertilization medium containing 1 mg/ml CS-C, it showed that the capacitated B pattern sperm cell was significantly ( p p