Acceleration of burn wound healing by mesenchymal stem cells from fetal membranes.

Objective:To assess the effect of fetal membrane-derived mesenchymal stem cells(MSCs) on full deep burn healing in rat.Methods:The term fetal membranes were treated with trypsin solution and the derived cells were cultivated with Amino-MAX Ⅱ media.The acelluar dermal matrix(ADM) was coated with different concentration of gelatin solutions as 3mg/ml,5mg/ml,7mg/ml and 9mg/ml.Cell solutions at 5×106/ml were seeded on coated and uncoated ADM respectively.Two hours later,the cells inadhere to the ADM were collected to calculate the efficiency of cell seeding.In experimental group,the cells(Passage 2 to 3) solutions at 5×106/ml were seeded on ADM coated by 7mg/ml gelatin,cultured for 3 to 5 days and then transplanted to the SD rat full-thickness burn wound.Control group I was transplanted with the ADM without cells and control group Ⅱ was not transplanted with ADM.The whole wound surface,wound contraction and the ratio of reepithelization area were calculated regularly.Tissue samples were harvested for histological examination in the fifth week.Results:Cells from fetal membranes were splindle-like in the majority,positive for the MSC surface antigen markers CD29 and CD73 and negative for those of hematopoietic cell CD14 and CD45.The coated and uncoated ADM with different concentration gelatin solutions didn't show statistical difference in the efficiency of cells seeding.Compared with control group,experimental group had longer transplant survival,earlier reepithelization,smaller contraction of wound surface and larger reepithelization area.Thicker regenerated epidermis and more apparent cuticular and capillary process were also observed in experiment group.Conclusion:MSCs from fetal membranes were successfully separated and cultured.Combining with ADM,the MSCs induced epidermis regeneration,accelerated the burn wound healing and inhibited the contraction of wound surface in the SD rats full-thickness burn wound model.