Procalcitonin detection kit, and method of measuring content of procalcitonin therewith

2016 
The invention especially relates to a procalcitonin detection kit, and a method of measuring content of procalcitonin therewith. The procalcitonin detection kit includes a reagent R1 and a reagent R2, wherein the reagent R1 is a reaction liquid formed by mixing various surfactants and used for promoting antibody-antigen specificity reaction, and the reagent R2 is a nano-latex particle buffer liquid of an anti-human procalcitonin antibody and contains 0.1-2% of nano-latex particles marked by the anti-human procalcitonin antibody. The kit is 0.12 ng/ml in detection sensitivity which is higher than analytic sensitivities of other like products. The nano-latex particle-type procalcitonin detection kit is high in sensitivity, good in specificity, high in accuracy and good in precision, wherein latex particles in different particle sizes are crosslinked with a polyclonal antibody to form conjugates in different particle sizes, which are then mixed according to different ratio, so that the kit is increased in linearity scope and is improved in detection sensitivity.
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