Quantitative gene expression of transcription factors T-bet and GATA-3 in rat lung allograft rejection

Objective To investigate the mRNA expression and significance of transcription factots T-bet and GATA-3 in rat lung acute allograft rejection.Methods Experimental animals were established and divided into normal group(n=5),allogeneic 3-day group(n=4)and allogeneic 5-day group (n=6).The expression of transcription factors T-bet and GATA-3 mRNA in rat lung allografts was detected by real-time PCR.Results The amplification efficiency of quantification of T-bet and GATA-3 with real-time PCR was 78%-85%.The Ct in intra-and interassay was＜0.15.Compared with normal controls (n=5),the expression had no significant change in allogeneic 3-day group:the expression of T-bet was increased(9.60±4.07 copies/106 copies),the expression of GATA-3 was decreased(0.82±0.04 copies/106 copies),and the ratio of T-bet and GATA-3 was increased(1.26±0.40 copies/106copies)in allogeneic 5-day group(P＜0.05).Conclusion The real-time PCR assay for quantification of T-bet and GATA-3 mRNA is efficient.special and reproductive.The increased T-bet and decreased GATA-3 are relative to acute rejcction.The expression of GATA-3 plays a maior role in acute rejection.Quantification of the ratio of T-bet/GATA-3 can be used as a more objective marker than T-bet or GATA-3 alone for estimation of the acute rejection of lung transplantation. Key words: Lung transplantation; Acute rejection; T-cell; GATA binding protein 3