Evaluation of antifungal activities of Grindelia robusta Nutt., Tanacetum praeteritum subsp. praeteritum and Alchemilla vulgaris L. extracts against plant pathogenic fungi

Purpose: Present study, we were carried out to evaluate the antifungal activities of extracts of Grindelia robusta Nutt., Tanacetum praeteritum subsp. praeteritum and Alchemilla vulgaris L. against Fusarium oxysporum f. sp. lycopersici, Botrytis cinerea Macrophamina phaseolina, Rhizoctania solani, Alternaria alternata, Cylindrocarpon destructans and Phytopthora infestans. Material and Methods: The screening of the antifungal potential of three extract forms from three different plant species was performed in vitro by using Agar-diffusion method on Potato Dextrose Agar media (PDA). One doses (1.0%, 1.5% and 2,0%) of the three diffent extracts were tested. Experimental results were obtained in three (n=3) replicates. Results: All plant extracts prepared in the study exhibited significant activity against plant pathogenic fungi. In particular, Tanacetum praeteritum subsp. praeteritum prevented the growth of micelles between 73.5 - 100% in five different fungal species, while Grindelia robusta Nutt. had a 100% effect on two of the four different fungus species. Conclusion: The extracts exhibited an antifungal activity against all the tested fungi. The analysis shown that the all extracts caused significantly inhibited the mycelium growth of fungi. This study paves the way for the development of new methods for environmentally friendly and economically viable phytosanitary practices.