GNRHR Gene Clone and Its mRNA Expression in Ovary of Gaoyou Duck

In order to explore the effect of GNRHR gene in forming procession of double-yolked egg,Gaoyou duck was chosed as experimental material,full length of duck GNRHR was cloned and its structure was analyzed. The mRNA expressions of GNRHR in high and low double-yolked egg duck populations at 258 and 330 days of age were detected by Real-time PCR repectively and the difference between the two genes were anlyzed. The results showed that the full length of GNRHR gene was 1 944 bp and composed of three exons and two introns. The mRNA expression of GNRHR gene in ovary of high double-yolked egg duck population was significant higher than that of low double-yolked egg duck population(P0.05).