Construction of genomic DNA library of Ceratocystis fimbriata by Lambda DASHII Phage.

According to comparison of extraction methods,genomic DNA of Ceratocystis fimbriata was extracted by CTAB/LiCl method.While 15kb DNA fragments were obtained by partial digestion of purified genomic DNA of C.fimbriata with restriction enzyme BamH I.Repurified 15kb DNA fragments were ligated with Lambda DASH II Phage DNA vector arms digested by BamH I.And a genomic DNA library of C.fimbriata was constructed successfully with XL1BlueMRA(P2) as host cell.The titer of the genomic DNA library was 4.12×105pfu/mL.