"ADPKD-omics": determinants of cyclic AMP levels in renal epithelial cells.

ABSTRACT The regulation of cyclic AMP (cAMP) levels in kidney epithelial cells is important in at least two groups of disorders, namely water balance disorders and autosomal dominant polycystic kidney disease (ADPKD). Focussing on the latter, we review genes that code for proteins that are determinants of cAMP levels in cells. We identify which of these determinants are expressed in the 14 kidney tubule segments using recently published RNA-seq and protein mass spectrometry data (“ADPKD-omics”). This includes G protein-coupled receptors, adenylyl cyclases, cyclic nucleotide phosphodieterases, cAMP transporters, cAMP binding proteins, regulator of G protein signaling (RGS) proteins, G protein-coupled receptor kinases, arrestins, calcium transporters, and calcium binding proteins. In addition, compartmentalized cAMP signaling in the primary cilium is discussed and a specialized database of the proteome of the primary cilium of cultured “IMCD3” cells is provided as an online resource ( https://esbl.nhlbi.nih.gov/Databases/CiliumProteome/ ). Overall, this paper provides a general resource in the form of a curated list of proteins likely to play roles in determination of cAMP levels in kidney epithelial cells and, therefore likely to be determinants of progression of ADPKD.