Recognition system of class II tRNA in Escherichia coli and yeast.

: The recognition system of class II tRNA, tRNA(Ser) and tRNA(Leu), in a yeast Saccharomyces cerevisiae was studied using T7 RNA polymerase transcription system. Yeast SerRS recognizes the long variable arm as in E. coli. However, the anticodon loop of tRNA(Leu), which has no effect on leucylation in E. coli, play a key role for recognition by LeuRS. Results suggest that the recognition style of yeast class II tRNA is substantially different from that of E. coli.