The effect of tranylcypromine on the invasion and migration of human colon cancer cells

Objective To investigate the effect of LSD1 on the invasion and migration of human colon cancer cells.Methods MAO inhibitor and gene silence was used to observe the effect of LSD1 on the invasion and migration of human colon cancer Lovo cells by scratch test and Transwell test in vitro.The gene expression of LSD1 was analyzed by RT-PCR and globle histone methylation in Lovo cells was detected by Western blot.Results At concentrations of 40,60 and 80 μmol/L MAO inhibitor Tranylcypromine (TCP),have no effect on the cell growth of human colon cancer Lovo cells (P ＞ 0.05).The scratch test showed that of TCP at 60 μmol/L effectively inhibited the migration of Lovo cells.The cell numbers of migration through the well were (69.7 ± 2.5) (60 μmol/L TCP group) and (222.7 ± 11.0) (control group) (P ＜0.05),while 60 μmol/L TCP didn't affect the expression of mRNA and protein level of LSD1 but increased the expression of H3K4me2 in Lovo cells.Conclusions MAO inhibitor TCP can inhibit the invasion and migration of human colon cancer Lovo cells at a concentration of 60 μmol/L. Key words: Colonic neoplasms; Cell migration assays; Tumor cells, cultured