BASIC PENTACYSTEINE2 negatively regulates osmotic stress tolerance by modulating LEA4-5 expression in Arabidopsis thaliana.

Osmotic stress substantially affects plant growth and development. Study of plant transcription factors involved in osmotic stress can enhance our understanding of the mechanisms of plant osmotic stress tolerance and how the tolerance of plants to osmotic stress can be improved. Here, we identified the specific function of Arabidopsis thaliana BARLEY B RECOMBINANT/BASIC PENTACYSTEINE transcription factor, BPC2, in the osmotic stress response. Phenotypic analysis showed that loss-of-function of BPC2 led to an increase in osmotic stress tolerance in the seedling growth stage. Physiological analysis showed that mutation of BPC2 in Arabidopsis alleviated osmotic-induced increases in H2O2 accumulation, the malondialdehyde (MDA) content, and percent electrolyte leakage. BPC2 was localized in the nucleus. RNA-seq and qRT-PCR analysis showed that BPC2 could negatively regulate the expression of late embryogenesis abundant (LEA) genes (LEA3, LEA4-2, and LEA4-5). Further analysis showed that BPC2 could directly bind to the promoter of LEA4-5 in vitro and in vivo. Overexpression of BPC2 enhanced hypersensitivity to osmotic stress in the seedling growth stage. Overexpression of BPC2 led to decreases in LEA4-5 expression and aggravated osmotic-induced increases in H2O2 accumulation, the MDA content, and percent electrolyte leakage. Overall, our results indicate that BPC2 negatively regulates LEA4-5 expression to modulate osmotic-induced H2O2 accumulation, the MDA content, and percent electrolyte leakage, all of which affect the osmotic stress response in Arabidopsis thaliana.