SDS-PAGE study on photooxidation damage of lysozyme induced by riboflavin

The photooxidation damage of lysozyme under 315–375 nm irradiation in the presence of riboflavin was studied by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Indications showed that the mechanisms and products of oxidative damage were relative to the concentration of riboflavin, the time of irradiation and the ambience. The type I process was examined in a nitrogen saturated solution, whereas both type I and type II were observed in an aerobic atmosphere and type II was the dominant process. The study also suggested that antioxidants, such as melatonin, can reduce the damage of lysozyme effectively.