Detection of grapevine rupestris stem pitting associated virus occurred in Shandong province.

In order to investigate the infection situation of grapevine rupestris stem pitting associated virus(GRSPaV)in Shan-dong province,298 grapevine samples of 58 cultivars were collected from major grape-growing areas and germplasm collec-tion plots of grapevines and tested for GRSPaV by plate trapped antigen indirect enzyme-linked immunosorbent assay(PTA-ELISA) and Dot -ELISA with a polyclonal antibody against prokaryotic expressed coat protein of GRSPaV.The results showed that 125 grapevine plants were positive to GRSPaV and the percentage was 41.95%.The positive samples involved 43 cultivars and the percentage was 74.14%.With 3 pairs of designed primers,anticipated fragments,340 bp for the helicase gene,842 bp fragment for CP gene and 499 bp fragment for RdRp gene,were amplified successfuly by RT-PCR from the samples showing positive reaction in ELISA tests.These results provide valuable information on the infection situation of the GRSPaV in Shandong province.