Olive leaf polyphenols alleviate oxidative stress and improve mitochondrial function in high glucose-induced 3T3-L1 hypertrophic adipocytes

Recent scientific evidences show that polyphenols derived from the olive tree leaf ( Olea europaea ) may have applications in obesity-related pathologies through AMPK activation. We aimed to explore the effect of olive leaf extract (OLE) on lipid accumulation, oxidative stress and mitochondrial potential in adipocyte model. In the present work, we used a glucotoxicity-induced insulin-resistant hypertrophic 3T3-L1-adipocytes model. Intracellular ROS generation and mitochondrial function was measured by ROS-sensitive fluorescent probes. Our results indicate that OLE showed the capacity to reduce triglyceride accumulation in hypertrophic adipocytes. In addition, the extract exhibited a dose-dependent decrease of intracellular ROS generations as well as restored the mitochondrial membrane potential in adipocytes. Therefore, olive leaf polyphenols may become for the dietary intervention focused on the management of obesity-associated disturbances.