The determination of flavonoids in Wikstroemia indica C. A. Mey. by liquid chromatography with photo-diode array detection and negative electrospray ionization tandem mass spectrometry

RATIONALE Flavonoids in the medicinal plant Wikstroemia indica C. A. Mey. are present in trace amounts and found in complex matrices. An efficient and sensitive method is necessary for the rapid identification of such biomolecules. METHODS Flavonoids were extracted with methanol via ultrasonic-assisted extraction and analyzed by liquid chromatography with photo-diode array detection and tandem mass spectrometry. The extract was analyzed and compounds were identified using negative electrospray ionization data-dependent tandem mass spectrometry. RESULTS The results confirmed the presence of three flavonoid compounds, seven biflavonoid compounds, and one coumarin-like compound, daphnoretin, in the extracts of different plant parts of W. indica. The method detection limit was evaluated down to 5 µg/g using kaempfol as a reference standard. CONCLUSIONS The proposed method offers a rapid and reliable analysis for the determination of flavonoids in medicinal plants. Copyright © 2012 John Wiley & Sons, Ltd.