A Simple and Cost Effective Turn off Fluorescence Sensor for Biliverdin and Bilirubin Based on L-Cysteine Modulated Copper Nanoclusters

The present article reports the efficiency of L-cysteine modulated copper nanoclusters (L-cys-CuNCs) as a fluorescent probe for the selective determination of naturally occurring bile pigments biliverdin (BVD) and bilirubin (BLR). These pigments were found to quench the fluorescence of L-cys-CuNCs through static processes. Under optimized conditions, the proposed strategy permitted the quantification of BVD and BLR in the range 4.00 × 10−5 to 5.00 × 10−7M and 1.00×10−5 to 1.00×10−6 M respectively with limits of detection 2.33 × 10−7M and 2.29 × 10−7 M. The practical utility of the developed sensor have been investigated in spiked blood and urine samples.