Making Drosophila lineage–restricted drivers via patterned recombination in neuroblasts

In this Technical Report, the authors describe a new technique for the unambiguous lineage tracing of specific Drosophila neuroblasts. This methodology involves the use of lineage-restricted drivers and a modification to GAL4 expression such that it is now permanent and heritable to all descendant cells, directing reporter expression based on neuroblast identity rather than terminal neuronal characteristics.