Anticonvulsant And Antioxidant Actions Of Some Dibenzo-α-Pyrone Derivatives In Pentylene -Induced Kindling Model In Mice

Objective: The present study was carried out to investigate the effect of Dibenzo-α-pyrone derivatives on the course of pentylenetetrazole (PTZ)-induced chemical kindling and oxidative stress markers in PTZ-kindled mice. Methods: Kindling was induced by repeated injections of a subconvulsive dose of PTZ (25mg/Kg, i.p.) on alternate days for 5 weeks or until stage 5 of the seizure score was evoked on three consecutive administrations. Butylamine, Diethylamine and Pyrrolidine Derivatives of Dibenzo-α-pyrone were administered daily in three doses (10, 20 and 40mg/kg) per orally (p.o.) along with alternate day PTZ. Following PTZ kindling , oxidative stress parameters , i.e. levels of malondialdehyde (MDA) and reduced glutathione (GSH), were assessed in isolated homogenized whole brain tissue. Results: PTZ treatment progressively increased the seizure score in control mice. Biochemical analysis revealed a significant increase in MDA levels and decreased GSH levels in the brain homogenate of PTZ-kindled mice. Daily treatment with Butylamine, Diethylamine and Pyrrolidine Derivatives of Dibenzo-α -pyrone in doses of 20 and 40mg/kg significantly decreased the PTZ-induced seizure score. However, a low dose (10mg/kg) failed to improve the seizure score. Pretreatment of derivatives in all doses showed an ameliorating effect on biochemical alteration induced by PTZ treatment. Conclusion: The present study indicate the potential anticonvulsant activity of Dibenzo- α-pyrone derivatives against PTZinduced kindling in mice.