Analysis of carbamazepine serum by differential pulse voltammetry (DPV) and comparison with fluorescence polarization immunoassay (FPIA): an animal study

Few studies have investigated the value of using an electrochemical method with dropping mercury electrodes to determine carbamazepine level. However, the toxic mercury can result in environmental pollution. In our previous study, we successfully developed a differential pulse voltammetric (DPV) method using a glassy carbon electrode instead of a dropping mercury electrode for the determination of carbamazepine level in standard solutions. In this study, we used the DPV method to determine the serum level of carbamazepine in rabbits. Blood samples were obtained from rabbits which had been fed carbamazepine. The serum concentration of carbamazepine from DPV using glassy carbon electrode and fluorescence polarization immunoassay (FPIA) technique was compared using a correlation test. In addition, DPV and FPIA techniques in carbamazepine detection were evaluated for precision, linearity, and detection limits using a standard solution. The correlation between the carbamazepine concentrations from DPV compared with those by FPIA was good (RSQ = 0.998). In addition, the coefficient of variation (CV) for the DPV technique and the FPIA technique were low. The precision and detection limit for both methods were satisfactory. The DPV method using a glassy carbon electrode can be a potential method for the determination of carbamazepine in serum.