Effects of Total Flavones from Bidens Bipinnata L.on Platelet Aggregation in vitro

Objective To investigate the effects of total flavones from Bindens bipinnata L.on rabbit platelet aggregation. Methods Platelet aggregation was induced by adenosine diphoshate(ADP) or thrombase(Thr),the inhibitory effects and dose-dependent relationship of total flavones from Bindens bipinnata L.were observed in vitro by turbidimetric method. Results The high and middlle dose of total flavones from Bindens bipinnata L.significantly suppressed the maximum platelet agglutination induced by ADP(P0.01),while the blocking rate of blood platelet aggregation was 45.82% and 28.65%,respectively.Additionally,these two doses of total flavones also demonstrated a strong restriction on the maximum platelet agglutination induced by Thr(P0.01),in which the curbing rate of blood platelet aggregation was reached to 48.36% and 33.82%,respectively.Total flavones exhibit a dose dependent manner. Conclusion Total flavones from Bindens bipinnata L.possesses powerful inhibitory abilities against the platelet aggregation induced by ADP and Thr in vitro.