APOPTOSIS INDUCTION OF EPIFRIEDELINOL ON HUMAN CERVICAL CANCER CELL LINE
2017
Background: Present investigation evaluates the antitumor activity of epifriedelinol for the management of cervical cancer
by inducing process of apoptosis.
Methods: Human Cervical Cancer Cell Line, C33A and HeLa were selected for study and treated with epifriedelinol at a
concentration of (50-1000 μg/ml). Cytotoxicity of epifriedelinol was estimated by MTT assay and induction of apoptosis
was assessed by estimating the activity of caspase 3, 8 and 9 enzyme, apoptosis assay and translocation of cytochrome c.
Moreover an expression of several proteins that plays role in the apoptosis process was estimated by western blot method.
Results: Result of the study suggested that treatment with epifriedelinol significantly decrease the viability count of
cancerous cell in a dose perndent manner and also enhances the formation of oligonucleosome in both the cell lines.
However activity of caspase enzymes and translocation of cytochrome c were enhanced after treatment with epifriedelinol.
It was also observed that epifriedelinol treatment alters the ratio of pro-apoptotic to anti-apoptotic proteins and enhances the
expressions of inhibitor of apoptosis proteins (IAP).
Conclusion: Result of our study proves the anticancer activity of epifriedelinol in cervical cancer by inducing apoptosis as
treatment with it enhances the production of oligonucleosomes, translocation of cytochrome c and activity caspase
enzymes.
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