SMALL, COST-EFFICIENT STOPPED-FLOW DEVICE

Conventional Stopped-Flow devices often need high sample volumes, display complex designs, take up much space and are very expensive in purchasing and maintenance. Here, a powerful microfluidic based Stopped-Flow system is described that is flexible in application, simple, easy to handle, and cost-efficient in terms of acquisition and operation. With a dead time of just 7 ms, the ability of kinetic measurements in absorption as well as fluorescence mode over a wide wavelength range, and the monitoring of intrinsic protein fluorescence without additional dyes at 280 nm, this small system is competitive to commercial available systems.