Mechanism of Eif6 Release from the Nascent 60S Ribosomal Subunit

During eukaryotic ribosome biogenesis, the nascent 60S subunit is activated by SBDS and GTPase EFL1. Cryo-EM and mutational analyses reveal how SBDS–EFL1 evicts antiassociation factor eIF6 from 60S and explain the effects of disease-related SBDS mutations.