Study on Enhancement of Antibacterial Character of Bacillus Licheniformis 01101107 Treated with Mutant Inducers

This study took the antagonistic bacterium as the research object.Based on the character of resisting common pathogen of this bacterium,and taking advantage of the mutagenesis effect of acredine orange,the antagonistic bacterium was induced to mutate.Then,the mutant bactrium strain of high antifungal activity was obtained by screening the mutant colony borrowing the piece of angragar inhibition zone method of single bacterium colony.Lastly,the genetic stability,efficiency value of the bacterium resistance and the span of the bacterium it resisted were measured by filter paper inhibition zone method,MTT-CCl4 extraction method,slab counting method and turbidimetry method,and it was comfirmed as highly stable,highly antibacterial and broad in antibacterial span,and it could be considered to be untilized in pharmaceutical or in gene engineering field as target bacterium.