Raman spectra and discrimination of NPC cell line CNE1 and normal nasopharyngeal cell line NP69
2009
As a non-destructive and non-invasive technique, Raman spectroscopy (RS) plays an important role in the
field of biomedical research. Great progress has been made in the research of biological samples from cellular
level to macro-tissues. In this letter, advances of RS in tumor cells and some statistic algorithm developed in
recent years for cancer differentiation and diagnosis are introduced. Also, Raman spectra of Nasopharyngeal
Carcinoma (NPC) cell line CNE1 and normal nasopharyngeal cell line NP69 are acquired by confocal Raman
micro-spectroscopy system. Raman bands are analyzed and compared to investigate the differences and
relationship between CNE1 and NP69, Principle Components Analysis (PCA) is used to classify CNE1 and
NP69 accurately with an accuracy of 100%. Comparing with CNE1, a blue-shift is observed in NP69 cells at
band 936cm -1 and 2935cm -1 which are assigned to C-C stretch and CH 3 stretching, respectively. Meanwhile, a
red-shift is observed at 1338cm -1 assigned to A, G and C-H deformation vibration of protein. The results show
that Raman spectroscopy has its potential and reliability to be one of the diagnostic methods for NPC and at
the same time can provide valuable information for cancer early diagnosis.
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