Method for simultaneously measuring 16 sulfonamides in beewax by high performance liquid chromatography tandem mass spectrometry

The invention relates to an inspection and quarantine method, and particularly relates to a method for simultaneously measuring 16 sulfonamides in beewax by high performance liquid chromatography tandem mass spectrometry. According to the method provided by the invention, normal hexane is adopted for pre-dissolution, a formic acid aqueous solution is extracted, an MCX solid phase extraction column is used for purification, an Agilent Eclipse Plus-18 column (100mm times 2.1mm, 3.5 microns) is used for separating, and an electrospray ion source positive ion multi-reaction monitor (MRM) mode tandem mass spectrum is used for measuring. The 16 sulfonamides, provided by the invention, respectively have better linear relation in a range of 2ng/mL to 50 ng/mL, and the correlation coefficients are larger than 0.995. Under the adding levels of 2.0 mu. g/kg, 5.0 mu. g/kg and 10.0 mu. g/kg, the recovery rate of the 16 sulfonamides in a practical sample is 65 to 127 percent, the relative standard deviation is (RSD, n is equal to 6) is 2.9 to 13.5 percent, and the limit of quantitation (S/N is more than 10) is 2.0 mu. g/kg. The method is quick, sensitive, accurate, and suitable for qualitative analysis and quantitative analysis of the residual of the 16 sulfonamides in a daily beewax sample.