Continuous butanol fermentation from inexpensive sugar-based feedstocks by Clostridium saccharobutylicum DSM 13864

Abstract Corn stover hydrolysate (CSH) and cane molasses were studied for butanol fermentation by Clostridium saccharobutylicum DSM 13864 in continuous fermentation. Using cane molasses as substrate, solvent of 13.75 g/L (butanol 8.65 g/L) and productivity of 0.439 g/L/h were achieved in a four-stage continuous fermentation at a gradient dilution mode of 0.15–0.15–0.125–0.1 h −1 . In continuous fermentation using CSH as substrate, total solvent titer of 11.43 g/L (butanol 7.81 g/L) and productivity of 0.429 g/L/h were reached at a dilution rate of 0.15 h −1 , and the steady process was continuously operated for 220 h without compromise in solvent titer.