Therapeutic protein analysis with the Agilent 2100 Bioanalyzer
2010
The Agilent 2100 Bioanalyzer is a microfluidic system for the electrophoresis-based analysis of biomolecules. In recent years it has developed into a key platform for routine QA/QC of protein samples by supporting three distinguished protein analysis kits (1): Protein 80 kit, for protein analysis in the low • molecular weight range Protein 230 kit, for general protein analysis up to • 230 kDa High Sensitivity Protein 250 kit, for picogram sensi• tivity and lowest level of impurity detection Besides the ease of use of prevalidated reagents, this protein kit portfolio offers a number of advantages over techniques like slab-gel electrophoresis or standard CESDS methods: Wide molecular weight range, from 5 kDa to 250 kDa • Broad protein loading capacity from pg/μL to μg/μL • Protein detection with up to four orders of magnitude • in linear dynamic range Minimal sample consumption • Electronic data • CFR 21 Part 11 compliance • This Technical Note compares the general performance features of the three protein analysis kits and provides guidance for selecting the kit best suited for the sample type at hand.
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