Study on polymorphism of chemokine receptor alleles influencing genetic susceptibility to HIV-1 infectionin

Objective Mutant frequency and polymorphism of HIV-1 res istance CCR-61I and SDF1-3′ A alleles were investigated in the population fro m Jinan Shandong province.Methods The study population included 234 subjects from J inan city.They came from Shanghe and Zhangqiu county;and none was HIV-1 positiv e .The genotypes of the two mutations were respectively detected by PCR/RFLP(restr iction fragment length polymorphism)assay with the digestion of restriction endo nuclease Bsa BI and Msp I for CCR2-64I and SDF1-3′ A mutation.Results The frequency of the mutant alleles were:20.25% for CCR2-64I and 21.00% for SDF1-3′ in Jinan city,Sha ndong province.Conclusion There were high frequencies of CCR2-64I and SDF1-3′ A alleles in Jinan population.The genetic susceptibi lity to HIV-1 infection and AIDS disease progression was low in Jinan city.